Hemocytometer Cell Count
Cells per mL from squares counted; optional trypan blue viability percent.
Example
You enter
- Total cells counted 240
- Squares counted 4
- Dilution factor 2
- Dead cells (optional, trypan blue) 12
You get
- Avg per square 60
- Cells per mL 1200000
- Viability 95
Checked against published worked examples from: Improved Neubauer hemocytometer (project bundled).
Details, formula, and sources
Formula or tablecells/mL = (total_cells / squares_counted) * 10^4 * dilution_factor. viability_pct = (total - dead) / total * 100.
Source and editionStandard improved Neubauer hemocytometer; each large corner square = 1 mm x 1 mm x 0.1 mm = 0.1 uL.
Free accessNo code citation required (cell-counting convention).
What governsVerify protocol against your lab's SOP before pipetting. A miscalculated dilution can ruin a run or a sample.
API field namestotal_cells_counted, squares_counted, dilution_factor, dead_cells, avg_per_square, cells_per_mL, viability_pct
- Chamber type improved Neubauer (1/10 mm depth)convention; older Neubauer / Burker chambers differ
- Counting method include cells touching top + left edges, exclude bottom + right (L-rule)convention; user is responsible for consistency