Michaelis-Menten Enzyme Kinetics
Reaction velocity and percent of Vmax from Vmax, Km, and substrate concentration (v = Vmax[S]/(Km+[S])).
Example
You enter
- Vmax (maximum velocity) 100
- Km (substrate at half Vmax) 25
- Substrate concentration [S] 25
You get
- Velocity 50
- Percent of Vmax 50
Checked against published worked examples from: Enzyme kinetics (Michaelis-Menten equation).
Details, formula, and sources
ScopeKm is the substrate at half Vmax -- an affinity proxy (low Km = high affinity), not a rate; the hyperbola never reaches Vmax.
Formula or tablev = Vmax x [S] / (Km + [S]); percent_vmax = v / Vmax x 100. At [S] = Km, v = Vmax / 2.
Source and editionThe Michaelis-Menten equation (enzyme kinetics), by name.
Free accessStandard biochemistry enzyme-kinetics relation; the assay conditions govern.
What governsEstimate. AHJ and licensed professional govern.
API field namesvmax, km, substrate, velocity, percent_vmax
- Steady state substrate far in excess of enzyme; single substrate, no cooperativity or inhibitionMichaelis-Menten