Serial Dilution Planner
Per-tube transfer volume, per-tube diluent volume, and resulting concentration at each step.
Example
You enter
- Starting concentration 1
- Dilution factor (>1) 10
- Volume per tube (L) 0.001
- Number of steps 5
You get
- Transfer volume per step 0.0001 L
- Diluent volume per tube 0.0009 L
- Dilution factor 10
- Volume per tube 0.001
Checked against published worked examples from: American Society for Microbiology.
Details, formula, and sources
Formula or tabletransfer_volume = volume_per_tube / dilution_factor. diluent_volume = volume_per_tube - transfer_volume. concentration[i] = starting / dilution_factor^i.
Source and editionStandard serial-dilution method. First principles.
Free accessNo code citation required.
What governsVerify protocol against your lab's SOP before pipetting. A miscalculated dilution can ruin a run or a sample.
API field namesstarting_concentration, dilution_factor, volume_per_tube, number_of_steps, transfer_volume, diluent_volume
- Equal volume per tube all tubes use the same volumeconvention; varied-volume protocols are user-customized