Lab Dilution and Serial Dilution

The missing concentration or volume in a dilution, plus each step of a serial dilution.

Run the calculator

Example

You enter

Stock C1 1000
Final C2 50
Final V2 100
Mode single

You get

C1 1000
V1 5
C2 50
V2 100
Diluent / final concentration Diluent 95

Details, formula, and sources

C1V1 = C2V2 missing-side solve; serial dilution series with per-step concentrations.

C1V1 = C2V2 single-mode missing-side solve. Serial-mode: each step divides by the dilution factor; final concentration = C1 × DF^(-n_steps).

Standard Methods for the Examination of Water and Wastewater (APHA / AWWA / WEF), 24th edition by name.

Standard Methods licensed; serial-dilution principles free at most NIH and university lab-protocol pages.

Estimate. Operator of record and primacy agency govern.

Field names used by the API: c1, c2, v2, mode, v1, diluent

Volumetric accuracy Class A volumetric glassware (≤ 0.1% error) or calibrated pipettesStandard Methods 1010

Details, formula, and sources

C1V1 = C2V2 missing-side solve; serial dilution series with per-step concentrations.

C1V1 = C2V2 single-mode missing-side solve. Serial-mode: each step divides by the dilution factor; final concentration = C1 × DF^(-n_steps).

Standard Methods for the Examination of Water and Wastewater (APHA / AWWA / WEF), 24th edition by name.

Standard Methods licensed; serial-dilution principles free at most NIH and university lab-protocol pages.

Estimate. Operator of record and primacy agency govern.

Volumetric accuracy Class A volumetric glassware (≤ 0.1% error) or calibrated pipettesStandard Methods 1010

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