Ligation Insert:Vector Molar Ratio
Insert mass to add for a target insert:vector molar ratio (insert_ng = ratio x insert_len/vector_len x vector_ng), plus each in picomoles at 650 g/mol per bp. A short insert needs less mass, not equal mass; equal masses over-represent small fragments.
Formula and source
insert_ng = ratio x (insert_length / vector_length) x vector_ng; vector_pmol = vector_ng / (vector_length x 650) x 1e3; insert_pmol = ratio x vector_pmol.
Ligation insert:vector molar-ratio setup (standard molecular cloning), by name.
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