PCR Master Mix
Per-component master-mix volume for N reactions with a pipetting fudge factor.
Example
You enter
- Number of reactions 24
- Fudge factor (%) 10
- Components (name,uL_per_reaction per line) name 2x Master Mix, per reaction 12.5, and 4 more
You get
- Total per reaction 25
- Total master mix volume (uL) 660
- Scaling factor 26.4
Checked against published worked examples from: Lab Manager.
Details, formula, and sources
Formula or tablescaling_factor = number_of_reactions * (1 + fudge_factor_pct / 100). component_total = component_per_reaction * scaling_factor.
Source and editionStandard master-mix arithmetic. Pipetting fudge factor (default 10%) accounts for dead-volume losses.
Free accessNo code citation required.
What governsVerify protocol against your lab's SOP before pipetting. A miscalculated dilution can ruin a run or a sample.
API field namesnumber_of_reactions, fudge_factor_pct, components, total_per_reaction, total_master_mix, scaling_factor
- Fudge factor default 10% (typical for 20-50 reactions)convention; lower for >100 reactions, higher for <12